Mouse Anti-FCGR2B Recombinant Antibody (TAB-036WM)
CAT#: TAB-036WM
This product is a recombinant mouse antibody that recognizes Fc-gamma-R2B (Fc fragment of IgG receptor IIb). The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-FCGR2B Antibody (TAB-036WM) in WB
Western blot analysis of TAB-036WM was performed by loading Human FCGR2B Protein (His Tag) in reduced condition Lane 1, Lane 2, Lane 3 (0.1 μg, 0.3 μg, 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with TAB-036WM (2 μg/mL) and HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000). Chemiluminescent detection was performed.
DB
Figure 2 Mouse Anti-Human FCGR2B Antibody (TAB-036WM) in DB
Dot Blot analysis of TAB-036WM was performed by coating with Human FCGR2B Protein (His Tag).
TAB-036WM incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 6000)
ELISA
Figure 3 Mouse Anti-Human FCGR2B Antibody (TAB-036WM) in ELISA
ELISA analysis of TAB-036WM was performed by coating with Human FCGR2B Protein (His Tag). Then blocked with BSA and incubated with TAB-036WM. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 5000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Normal Tissue
Figure 1 Placenta
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Hofbauer cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining: Medium
Intensity: Moderate
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining: High
Intensity: Strong
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7796/18532_A_3_7.jpg
Normal Tissue
Figure 2 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/7796/18532_A_8_8.jpg
RNA Expression
Figure 3 RNA cell line category: Cell line enhanced (Daudi, HMC-1, NB-4, RPMI-8226)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000072694-FCGR2B
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human Fc-gamma-R2B (Fc fragment of IgG receptor IIb)
- Species Reactivity
- Human
- Applications
- ELISA, FC, Inhib
- Related Disease
- Human Primary Ovarian, Breast Carcinoma
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- FCGR2B; Fc fragment of IgG, low affinity IIb, receptor (CD32); CD32; FCG2; CD32B; FCGR2; IGFR2; low affinity immunoglobulin gamma Fc region receptor II-b; CDw32; fcRII-b; Fc gamma RIIb; fc-gamma-RIIb; fc-gamma RII-b; igG Fc receptor II-b; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32)
- Gene ID
- 2213
- UniProt ID
- P31994
REVIEWS AND Q&AS
CITATIONS
RESOURCES
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Customer Review
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Problem Solved Quickly
The antibody arrived on time, just in time for us to use it in a crucial investigation. Our project was on schedule and we saved a ton of time thanks to the prompt delivery.
Speedy Shipping
performed admirably even under demanding circumstances, with no non-specific binding. Having a tool this dependable for our virus detection assays is fantastic.
No Generalized Binding
The fact that this antibody may be modified to fit different experimental and prototyping procedures without losing its effectiveness says a lot about its robustness and versatility.
User 188*****2807.May,24
Western Blot for TAB-036WM
Clear band observed by WB.
Q&As
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How can I select the lysis buffer that will work best for my samples?
A: Based on compatibility with the intended usage downstream, choose a lysis buffer. Membrane-bound receptors are commonly treated using RIPA and NP-40 buffers.
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What details are included in the antibody datasheet?
A: The datasheet includes information on concentration, suggested dilutions, published references, storage conditions, and proven applications to help you use the antibody effectively.
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Is there a particular buffer that is suggested for reconstitution of antibodies?
A: Sterile PBS or a commercial antibody diluent are typically recommended for reconstitution. The specific buffer should be specified in the product datasheet.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# TAB-036WM, RRID: AB_3111773)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1086z-S(P) | Recombinant Anti-Human FCGR2B Antibody scFv Fragment | FC, ELISA, FuncS | scFv |
| HPAB-0180-WJ-S(P) | Human Anti-FCGR2B Recombinant Antibody (clone NOV0281); scFv Fragment | ELISA, FC, WB | Human scFv |
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| HPAB-1697-FY-F(E) | Mouse Anti-FCGR2B Recombinant Antibody; Fab Fragment (HPAB-1697-FY-F(E)) | ELISA, IF | Mouse Fab |
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| CAT | Product Name | Application | Type |
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| TAB-267CL | Human Anti-FCGR2B Recombinant Antibody (TAB-267CL) | FC, WB | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-034WM | Human Anti-FCGR2B Recombinant Antibody (TAB-034WM) | ELISA, FC, WB | Human IgG |
| TAB-034WM-S(P) | Human Anti-FCGR2B Recombinant Antibody; scFv Fragment (TAB-034WM-S(P)) | ELISA, FC, WB | Human scFv |
| TAB-034WM-F(E) | Human Anti-FCGR2B Recombinant Antibody; Fab Fragment (TAB-034WM-F(E)) | ELISA, FC, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-035WM | Mouse Anti-FCGR2B Recombinant Antibody (TAB-035WM) | ELISA, FC, Inhib | Mouse IgG |
| TAB-035WM-S(P) | Mouse Anti-FCGR2B Recombinant Antibody; scFv Fragment (TAB-035WM-S(P)) | ELISA, FC, WB | Mouse scFv |
| TAB-036WM-S(P) | Mouse Anti-FCGR2B Recombinant Antibody; scFv Fragment (TAB-036WM-S(P)) | ELISA, FC, WB | Mouse scFv |
| TAB-035WM-F(E) | Mouse Anti-FCGR2B Recombinant Antibody; Fab Fragment (TAB-035WM-F(E)) | ELISA, FC, WB | Mouse Fab |
| TAB-036WM-F(E) | Mouse Anti-FCGR2B Recombinant Antibody; Fab Fragment (TAB-036WM-F(E)) | ELISA, FC, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-037WM | Human Anti-FCGR2B Recombinant Antibody (TAB-037WM) | ELISA, FuncS | Chimeric (human/mouse) IgG |
| TAB-037WM-S(P) | Anti-FCGR2B Recombinant Antibody; scFv Fragment (TAB-037WM-S(P)) | ELISA, FC, WB | Mouse scFv |
| TAB-037WM-F(E) | Human Anti-FCGR2B Recombinant Antibody; Fab Fragment (TAB-037WM-F(E)) | ELISA, FC, WB | Chimeric Antibody (human/mouse) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-038WM | Human Anti-FCGR2B Recombinant Antibody (TAB-038WM) | ELISA, FuncS | Humanized Antibody |
| TAB-038WM-S(P) | Human Anti-FCGR2B Recombinant Antibody; scFv Fragment (TAB-038WM-S(P)) | ELISA, FC, WB | Humanized scFv |
| TAB-038WM-F(E) | Human Anti-FCGR2B Recombinant Antibody; Fab Fragment (TAB-038WM-F(E)) | ELISA, FC, WB | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| VS-0325-XY826 | Anti-FCGR2B Immunohistochemistry Kit | IHC | |
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